由 AV Lin 著作 · 2015 · 被引用 131 次 — This chapter provides the procedures required for carrying out indirect ELISA, one of the many forms of ELISA, to detect polystyrene-immobilized antigen.
Indirect ELISA is a technique that uses a two-step process for detection, whereby a primary antibody specific for the antigen binds to the target, and a labeled ...
Indirect ELISA Protocol · Methods and Principles from our Scientific Staff · Coating the Plate with Antigen · Blocking the Plates · Incubate with Primary ...
The indirect ELISA method has high sensitivity since more than one labeled secondary antibody can bind the primary antibody; it is more economical than the ...
An indirect ELISA is similar to a direct ELISA in that an antigen is immobilized on a plate, but it includes an additional amplification detection step. First, ...